Coding

Part:BBa_K2984033:Design

Designed by: Darius Rauch   Group: iGEM19_Humboldt_Berlin   (2019-10-16)


L1c-AR-ARS-YFP-Rbcs2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 953
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 268
    Illegal PstI site found at 953
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 953
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 953
    Illegal NgoMIV site found at 1430
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Secretion vector designed for transformation in C. reinhardtii and further cloning into a level 2 backbone (according to the MoClo standard) by GoldenGate cloning.


Source

The construct consists of the four different Level 0 parts AR (BBa_K2984025), ARS (BBa_K2984000), YFP (BBa_K2984017) and RbcS2 (BBa_K2984018). which were cloned into the level 1c backbone (BBa_K2984002) using the MoClo Standard.

References

Schroda, M., Blöcker, D., & Beck, C. F. (2000). The HSP70A promoter as a tool for the improved expression of transgenes in Chlamydomonas. The plant journal, 21(2), 121-131.

N. J. Patron, D. Orzaez, et al. „Standards for plant synthetic biology: a common syntax for exchange of DNA parts“. In: New Phytologist 208.1 (Oct. 2015), pp. 13–19. issn: 0028-646X.